TALOS+ is, as the original program TALOS, a program which predicts torsion angles using information derived from chemical
shift, amino acid type, and the area of the Ramachandran map where the residue is likely to reside in. This information is
compared to information stored in a large database; currently 200 proteins. The input data for this webserver are the
13CA, 13CB, 13C', 15N, 1HA and 1HN NMR backbone chemical shifts of the query protein, either in TALOS or in BMRB format.
TALOS+, in contrast to TALOS, includes a
neural network component whose output is used as an additional empirical term in the conventional TALOS database search.
The neural network component of the program relies on a well established computational framework that optimizes the
relation between a large number of input variables.
- Reference: Y. Shen, F. Delaglio, G. Cornilescu, and A. Bax. J. Biomol. NMR, 44(2009):213-223
In order to prevent assignment of backbone torsion angles to regions that are flexible, TALOS+ uses a flexibility
prediction program RCI to determine such regions. The methodology is recently described by Berjanskii and Wishart (J. Am. Chem. Soc. 127: 14970-14971).
- TALOS+ webserver
- Example of TALOS+ webserver output
- Questions? Problems? Suggestion?
Contact us via the Talos+ WeNMR Help Center
- References for use of the WeNMR TALOS server:
T.A. Wassenaar, M. van Dijk, N. Loureiro-Ferreira, G. van der Schot, S.J. de Vries, C. Schmitz, J. van der Zwan, R. Boelens, A. Giachetti, L. Ferella, A. Rosato, I. Bertini, T. Herrmann, H.R.A. Jonker, A. Bagaria, V. Jaravine, P. Guntert, H. Schwalbe, W.F. Vranken, J.F. Doreleijers, G. Vriend, G.W. Vuister, D. Franke, A. Kikhney, D.I. Svergun, R. Fogh, J. Ionides, E.D. Laue, C. Spronk, S. Jurka, M. Verlato, S. Badoer, S. Dal Pra, M. Mazzucato, E. Frizziero and A.M.J.J. Bonvin
"WeNMR: Structural Biology on the Grid."
J. Grid. Comp., 10, 743-767 (2012).